ethidium bromide disposal

Since ethidium bromide is used in conjunction with an ultraviolet light source, please review the Ultraviolet Radiation Safety Fact Sheet. Ethidium Bromide is a dye commonly used as a fluorescent tag when performing molecular biology techniques such as gel electrophoresis. Tel : +91 22 2687 2601 Fax : +91 22 2687 8241 Website : www.srlchem.com 2 - COMPOSITION, INFORMATION ON INGREDIENTS CAS# Chemical Name: % EINECS# 1239-45-8 Ethidium bromide 98% 214-984-6 This document establishes procedures for the safe handling and disposal of Ethidium Bromide (CAS# 1239-45-8, commonly abbreviated EtBr). Q - How should I manage and dispose of ethidium bromide waste? All ethidium bromide solutions and gels must be collected and treated as if it were a hazardous waste, unless the ethidium bromide has been removed by one of the methods listed below. UC San Diego researchers working with ethidium bromide must complete the Acutely Toxic hazard control plan . Answer - Ethidium bromide is a mutagen that requires special storage, handling, and disposal as waste. Submit for Chemical Waste Collection a blue "Contaminated Waste Label" - identify the materials as "Ethidium . The 10 mg/ml ethidium bromide stock solution can be diluted for gel staining or used as provided for CsC1 gradient protocols. with a price - $300 per 55-gallon drum.

Ethidium bromide is the most commonly used dye for DNA and RNA detection in gels. • pH neutral, aqueous solutions containing less than or equal to 5 ppm (mg/L . Don't forget to date the hazardous waste label with the date waste is first put into the container.

. Ethidium Bromide (EtBr) is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. Ethidium bromide (EtBr) is a powerful mutagen widely used in biochemical research laboratories for visualizing nucleic acids. It fluorescents a red-orange color under ultraviolet (UV) light and with increased fluorescence when bound to double-stranded DNA. EtBr is generally used in the laboratory dissolved in . EtBr is generally used in the laboratory dissolved in . Indicate on the Hazardous Waste label and the chemical waste disposal request form the concentration of ethidium bromide in solution. Ethidium bromide (C 21 H 20 BrN 3) is a potent mutagen used as a nucleic acid stain. Or would you like to test a free sample*? It is known to be toxic and mutagenic and may be fatal if swallowed and harmful if inhaled or absorbed through the skin. WASTE DISPOSAL PROCEDURES FOR GELS AND RUNNING BUFFERS CONTAINING NUCLEIC ACID STAINS/DYES Type of Unwanted Material Waste Disposal Procedures TAE, TBE, Tris/Glycine/SDS running buffers containing: Ethidium Bromide < 10ug/mL Drain disposal Ethidium Bromide > 10ug/mL Requires in lab deactivation or disposal by EHS Ethidium bromide is strongly mutagenic and is irritating to the eyes, skin, mucous membranes and upper respiratory tract. When one considers that the overhead charges on research grants pay for chemical waste disposal, it is clear that ethidium bromide disposal is an obscured, yet expensive and potentially avoidable cost of . Eyes, skin, mucous membranes, and upper respiratory tract may be . Ethidium bromide has UV absorbance maxima at 300 and 360 nm, and an emission maximum at 590 nm.

The compound forms fluorescent complexes by intercalation and these compounds are readily visible under ultraviolet (UV) light. Ethidium Bromide (EtBr; 3,8 diamino-5-ethyl-6-phenyl phenanthridinium bromide, dromilac, CAS #1239-45-8), commonly used in research laboratories as astain for the visualization of nucleic acids in EtBr is a dark red, crystalline, non-volatile solid, moderately soluble in water, which fluoresces

Ethidium bromide in an organic solvent solution can be bulked into a yellow waste carboy. Ethidium bromide (EtBr) or 3,8-Diamino-5-ethyl-6-phenylphenanthridinium is commonly used as a non-radioactive marker to stain DNA in order to identify and visualize nucleic acid bands in electrophoresis and other gel-based methods of nucleic acid separation. protection. Disposal Unit price, dose & cost per gel Ethidium Bromide 1 dsDNA ssDNA RNA PCR 290 nm 605 nm agarose acrylamide 0.2ng-0.5ng May be stored at room temperature; indefinite storage UV Transilluminator Polaroid 667 black & white Mutagenic with S9 activation of Salmonella TA98 and TA1537 strains LD 50, rat (oral): 1503 mg/kg (slightly toxic) Aquatic 7.19.1 Dry Ethidium Bromide Wastes, Including Gloves and Papers. Ethidium bromide (EtBr) is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other methods of nucleic acid separation. List price is being displayed temporarily. Ethidium Bromide / Acrylamide Waste. NOTE: Ethidium bromide is a chemical and should NOT be treated or labeled as a biohazard. Use absorbent materials if needed and dispose into a chemical waste container. P403 + P233 Store in a well-ventilated place. Section 10. We are currently unable to calculate your contract price for this item. Activated charcoal extraction of ethidium bromide is a hazardous waste volume reduction method, whereas a chemical .

Ethidium Bromide (EtBr) wastes are commonly collected from ARS laboratories. Ethidium bromide can be degraded chemically, or collected and incinerated. 1. UCCS.SOP 23 Ethidium Bromide Page 1 of 4 UCCS SAFE OPERATING PROCEDURE 23. It fluoresces a red-orange colour under ultraviolet light and with increased fluorescence when bound to double-stranded DNA.Typically EB is purchased in powder or solution form and is soluble in water. Though not a carcinogen, it behaves as a mutagen in the Ames Salmonella bioassay. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Uses advised against Food, drug, pesticide or biocidal product use. Ethidium Bromide (EtBr) Dye for DNA and RNA Detection. : AC170960000; AC170960010; AC170960050; AC170960250 CAS-No 1239-45-8 Synonyms Homidium bromide Recommended Use Laboratory chemicals. Ethidium Bromide. Contaminated packaging Dispose of as unused product. Ethidium bromide is the most commonly used dye for this type of DNA work and the most toxic. 1.2 These procedures must be followed by all who work with Ethidium Bromide. SDS-Ethidium Bromide Page 4 of 8 Methods and materials for containment and cleaning up Clean up spills immediately. ETHIDIUM BROMIDE 98% AR MSDS CAS: 1239-45-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: ETHIDIUM BROMIDE AR CAS#: 1239-45-8 Synonym: Homidium bromide Chemical Name: Ethidium Bromide AR Chemical Formula: C 21 H 20 BrN 3 Brand: OXFORD Details Of The Supplier Of The Safety Data Sheet :

Identification Product Name Ethidium bromide, 1% Solution/Molecular Biology Cat No. Ethidium Bromide Treatment and Disposal Ethidium bromide is a commonly used stain for identifying nucleic acids in electrophoresis gels. Requirement for researchers. Solid Ethidium Bromide This Bulletin describes the policy change and how it affects the way you manage your ethidium bromide waste.

Identification of the substance Ethidium bromide Article number 7870 Registration number (REACH) It is not required to list the identified uses be-cause the substance is not subject to registration according to REACH (< 1 t/a) Index No 612-278-00-6 EC number 214-984-6 CAS number 1239-45-8 It may be harmful by inhalation, ingestion, or skin absorption. Background Ethidium bromide (3,8 diamino-5-ethyl-6-phenyl phenanthridinium bromide, dromilac, CAS #1239-45-8), is a compound used in many UTK laboratories Ethidium bromide is available as a dark red, crystalline, non-volatile solid and is moderately soluble in water.

Turn-in all solutions containing ethidium bromide. Guidelines for Ethidium Bromide Disposal Ethidium bromide (EtBr) is commonly used as a marker for identifying and visualizing nucleic acid bonds in electrophoreses and in other methods of gel-based nucleic acid separation. One kit has the capacity to remove 500 mg of ethidium bromide from solutions (10 mg EtBr/bag). Overview Ethidium bromide (EtBr) is both an irritant and a mutagen and a frame shift mutagen, which inserts double-stranded DNA and RNA resulting in the inhibition of DNA synthesis. Use the following procedures when disposing ethidium bromide solutions, gels, and contaminated materials. Conditions for safe storage When you work with EtBr, you must wear a lab coat, chemical splash goggles, and nitrile gloves. Ethidium bromide (EtBr) requires extra precautions during use and disposal because of its highly toxic and mutagenic properties. ETHIDIUM BROMIDE USE AND DISPOSAL Introduction Although ethidium bromide (EtBr) is not regulated as a hazardous waste, its mutagenic properties may present a human health hazard if it is placed in the trash or poured down the sanitary sewer system. It causes eye and skin irritation.

Ethidium Bromide waste in concentrated or solid form is collected as hazardous waste and should not be flushed down the drain or disposed of in the trash. P304 + P340 + P311 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Description. Ethidium bromide is frequently used in molecular biology laboratories as a nucleic acid stain due to its ability to intercalate DNA and its fluorescent properties when exposed to ultraviolet light. Print. Ethidium Bromide Safe Handling, Disposal, and Emergency Procedures . Use the following procedures when working with or disposing EtBr Ltd. Andheri (East), Mumbai - 400 099. DOT (US) Not dangerous goods. Stability and Reactivity Section 11. GUIDE FOR ETHIDIUM BROMIDE USE AND DISPOSAL . Further, take appropriate step to minimize your exposure. Ethidium Bromide Disposal Aqueous Ethidium Bromide Stock Solutions Dispose of aqueous ethidium bromide solutions through Environmental Health and Safety (EHS) in plastic or glass containers. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques . Ethidium Bromide Greenbag™ Disposal Kit. More recently this wariness has been whipped up into a witch hunt by companies touting "safer" alternatives and disposal methods. As with all hazardous waste, waste solutions cannot be diluted or . Materials that do not fluoresce under UV light may be disposed of directly in the trash. List price is being displayed temporarily. All grossly contaminated materials such as gloves, wipes, etc. Ethidium bromide is commonly used in molecular biology laboratories for DNA identification in electrophoresis. SAFE HANDLING AND DISPOSAL OF ETHIDIUM BROMIDE (For assistance, please contact Environmental Health & Safety) Ethidium bromide is a nucleic acid stain. OCTOBER 2014 1 Environmental Health and Safety. Ethidium bromide (EtBr) is a powerful mutagen widely used in biochemical research laboratories for visualizing nucleic acids. Ethidium Bromide Waste Management Bulletin A new Standard Operating Procedure for ethidium bromide use and disposal was completed and introduced at Departmental/School Laboratory Safety Committees in 2010. Ethidium Bromide (EtBr) Disposal Ethidium bromide (EtBr) is a strong mutagen and a possible carcinogen or teratogen. ETHIDIUM BROMIDE DISPOSAL _____ Ethidium bromide (EtBr) is a strong mutagenthat is regulated as a "special waste" when disposed. Ethidium Bromide Greenbag™ Disposal Kit. Ethidium bromide, 1% Solution/Molecular Biology Revision Date 17-Jun-2021 Full text of Hazard Statements: see section 16 2.2. . ETHIDIUM BROMIDE: While not regulated as a "hazardous waste", the mutagenic properties of this substance may present a hazard if improperly disposed of down the drain or placed into the trash. ETHIDIUM BROMIDE 1. Ethidium bromide is a strong mutagen and EH&S recommends that it be deactivated before being sewered. romide (Mutagen) Ethidium Bromide forms Proper collection and disposal Dilute Aqueous Solutions Solutions containing less than 10 μg/ml ethidium IATA Not dangerous goods.

The following procedure permits the destruction of 100 ml of 0.5 mg/ml solution of ethidium bromide in TBE buffer, MOPPS buffer or cesium . : BP1302-10 Synonyms No information available Recommended Use Laboratory chemicals. EHS recommends the specific disposal procedures for ethidium bromide as follows: Charcoal Filtration/Absorption

Since ethidium bromide is used in conjunction with an ultraviolet light source, please review the Ultraviolet Radiation Safety Fact Sheet. Solid ethidium bromide powder and aqueous ethidium bromide solutions should be clearly labeled in a sealed container and given to Chemical Safety via the Surplus Chemical Pick-up Request for proper disposal. Treatment may be accomplished by extracting ethidium bromide from staining solutions using activated charcoal or by chemical neutralization to reduce the mutagenicity. This SOP provides guidance on the various disposal for EtBr solids and liquids.

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